gt; Thank you
> Dhiraj
>
>
> -
> From: CCP4 bulletin board on behalf of Nikolay
> Dobrev
> Sent: Thursday, February 3, 2022 11:53 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [External] Re: [ccp4bb] protease inhibitor and 3c protease
>
> Dear Dhi
Dobrev
Sent: Thursday, February 3, 2022 11:53 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [External] Re: [ccp4bb] protease inhibitor and 3c protease
Dear Dhiraj,
the 3C protease is from the Cysteine protease family.
Therefore for sure, you can add EDTA (if it is not indented for follow up IMAC
Dear Dhiraj,
the 3C protease is from the Cysteine protease family.
Therefore for sure, you can add EDTA (if it is not indented for follow up IMAC
purification) and that will inhibit most of the metalloprotease. Furthermore,
you can resort to Serine type protease inhibitors like PMSF or Aprotinin.
Hi
sorry for off topic question. does anyone know which protease inhibitors we
can include safely while cleaving with 3C protease?
Thank you
Dhiraj
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