Re: [ccp4bb] Fwd: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-05 Thread Andreas Forster
Hello Klaus, I think you should give Gerard some "Hofnarrenfreiheit". (That's a fine German word for you to figure out, Gerard.) He is certainly not an evil racist at heart. Andreas Klaus Piontek wrote: Greetings (or in "correct" Swiss German "Grüezi wohl", with Umlaut=vowel mutation) t

[ccp4bb] Fwd: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread Klaus Piontek
ROTECTED]> Sender: CCP4 bulletin board From: Gerard DVD Kleywegt <[EMAIL PROTECTED]> Subject: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data) Comments: To: Dirk Kostrewa <[EMAIL PROTECTED]> To: CCP4BB@JISCMAIL.AC.UK L

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Arti S. Pandey
We all begin at some point, and should help ourselves before we ask others for help. Shivesh's question shows he has not even tried looking at programs, initiate things on his own. I think such e mails should be discouraged. I found what Mark said quite funny. All my labmates were laughing.. Ar

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Eleanor Dodson
shivesh kumar wrote: Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.How should I process the data.Thanx for the help. Shivesh I think you should ask your local crystallographer for help. There are several programs ( MOSFLM in CCP4, DENZO, XDS, D*TREK ) but you

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Phil Evans
On 1 Mar 2007, at 10:46, James Whisstock wrote: Once you have integrated (run in ccp4 for MOSFLM), then you need to scale the data using, for example, SCALA. Have a look at the following tutorial for running scala. http://www.ccp4.ac.uk/courses/ECM2004/runningscala.pdf An updated versi

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread James Whisstock
Dear Shivesh Below I've tried to give you some broad ideas about where to look / read and some packages / approaches to try. For a start there is a very comprehensive tutorial on the ccp4 site together with the experiemental phasing roadmaps. http://www.ccp4.ac.uk/dist/examples/tutorial/html/h

Re: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread James Stroud
I have found in my usenet adventures that usenet mavens often point to this link before trying to sort out among themselves exactly what level of humor to take with dubious posts: http://www.catb.org/~esr/faqs/smart-questions.html Of course usenet mavens also complain a little too m

Re: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread Dirk Kostrewa
Goede dag Gerard, interesting that you think my last e-mail was addressed to you ;-)! Yes, this was my first moralizing posting on this board after some 16 years. A bit sense of humor is fine, but the last posting was simply too much. We shouldn't attack people personally or discriminate agains

[ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread Gerard DVD Kleywegt
gruzi dirk, come on - lighten up a little! your reply wasn't funny or helpful either - just moralising. (by the way - 't is a strange fact that whenever i get any negative reactions to my own postings they stem *exclusively* from any or all of these three countries: the us, germany and switzer

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Gerard Bricogne
Dear all, I wholeheartedly agree with Dirk. I was quite speechless yesterday when seeing one sarcastic reply after another being sent to this correspondent, who has probably been put off using this BB ever again. Its purpose is to help people who need to ask such questions, rather than to ser

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Miguel Ortiz Lombardia
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear ccp4bbers, I agree with Dirk. I have also noticed that much due to the way X-ray crystallography is evolving, a lot of students/early-postdocs find themselves "doing crystallography" in labs without a tradition in crystallography, even without "r

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Dirk Kostrewa
Hi Mark, although Shivesh's question was not very specific, and he should have clearly given some more informations about what he would like to know, he is probably a beginner in crystallography and simply asked for help on this board. Not everyone has always time or is always in the mood to

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Mark J. van Raaij
why don't you just send all your images to the ccp4bb, then we'll process them, solve the structure and publish it for you. And we might put you in the acknowledgements, if you are lucky. Mark On 28 Feb 2007, at 16:35, Jonathan Grimes wrote: Anastassis Perrakis wrote: On Feb 28, 2007, at 14:3

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Clemens Vonrhein
>On Feb 28, 2007, at 14:37, shivesh kumar wrote: > >Dear all, >I have a data set at 2.2A, of the selenomethionene labelled >protein.How should I process the data. Some hopefully useful remarks (fairly random and not complete and exhaustive): 1. make sure to mask out the backstop and beamstop hol

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Jonathan Grimes
Anastassis Perrakis wrote: On Feb 28, 2007, at 14:37, shivesh kumar wrote: Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.How should I process the data. Carefully ! Thanx for the help. Shivesh Tassos i am sure what tassos really meant was "Very Careful

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Petrus H Zwart
> Dear all, > I have a data set at 2.2A, of the selenomethionene labelled > protein.How should I process the > data. Properly

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Anastassis Perrakis
On Feb 28, 2007, at 14:37, shivesh kumar wrote: Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.How should I process the data. Carefully ! Thanx for the help. Shivesh Tassos

[ccp4bb] process SeMet labelled data

2007-02-28 Thread shivesh kumar
Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.Howshould I process the data.Thanx for the help. Shivesh