Hi,
What size is the protein and what detergent are you using? Depending
on the size of the protein and the size of the detergent micelles you
might very well concentrate the detergent during the protein
concentration. DDM for example is always concentrated if you have a
MWCO less than 10
2008/1/23, Zheng, Lei <[EMAIL PROTECTED]>:
>
> Hi ccp4ers,
>
>
>
> Sorry for this out-topic question:
>
> Recently we have a membrane protein expressed, after solubilized with
> detergent and purified from IMAC, the protein looks beautiful in SEC.
> However, it completely precipitates after the 2-
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] problem on protein precipitation
Hi ccp4ers,
Sorry for this out-topic question:
Recently we have a membrane protein expressed, after solubilized with
detergent and purified from IMAC, the protein looks beautiful in SEC.
However, it completely
Hi ccp4ers,
Sorry for this out-topic question:
Recently we have a membrane protein expressed, after solubilized with
detergent and purified from IMAC, the protein looks beautiful in SEC.
However, it completely precipitates after the 2-3 days storage in 4
degree. We supplement 2 mM DTT in the n
