Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-02-15 Thread sreetama das
Dear All,   Thank you for your replies: 2 different methods were suggested for the citrate buffer preparation over a small range of pH: 1. mixing citric acid with sodium citrate salts 2. mixing citric acid with NaOH we found the latter method easier for our purpose.

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread Edward A. Berry
Nick From: Roger Rowlett mailto:rrowl...@colgate.edu>> Reply-To: Roger Rowlett mailto:rrowl...@colgate.edu>> Date: Thursday, January 30, 2014 at 7:23 AM To: "CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>" mailto:CCP4BB@JISCMAIL.AC.UK>> Sub

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread Roger Rowlett
.edu>> Reply-To: Roger Rowlett mailto:rrowl...@colgate.edu>> Date: Thursday, January 30, 2014 at 7:23 AM To: "CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>" mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] preparation of

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread Shane Caldwell
to calculate >> volume of components while designing a tray as long as you tell it the >> concentrations. >> >> http://hamptonresearch.com/make_tray.aspx >> >> Nick >> >> From: Roger Rowlett >> Reply-To: Roger Rowlett >> Date: Thursd

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread David Schuller
RE citrate buffer preparation The Calbiochem buffers has some generally useful information about buffers; pKa and such. http://www.antibodybeyond.com/books/Calbiochem_Buffers_Booklet_CB0052_E.pdf http://wolfson.huji.ac.il/purification/PDF/Buffers/Calbiochem_Buffers_Booklet.pdf --

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread Katherine Sippel
while designing a tray as long as you tell it the > concentrations. > > http://hamptonresearch.com/make_tray.aspx > > Nick > > From: Roger Rowlett > Reply-To: Roger Rowlett > Date: Thursday, January 30, 2014 at 7:23 AM > To: "CCP4BB@JISCMAIL.AC.UK" > Subject: R

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread Daniel Picot
...@colgate.edu>> Reply-To: Roger Rowlett <mailto:rrowl...@colgate.edu>> Date: Thursday, January 30, 2014 at 7:23 AM To: "CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>" mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] preparation of citrate buffe

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread Schnicker, Nicholas J
mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] preparation of citrate buffer pH3-6.5 The easiest way to produce repeatable conditions is to titrate a stock solution (say 1M) of citric acid with NaOH to the desired pH and use that to mix your screen. That's what Hampton does anyway. I

Re: [ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-30 Thread Roger Rowlett
The easiest way to produce repeatable conditions is to titrate a stock solution (say 1M) of citric acid with NaOH to the desired pH and use that to mix your screen. That's what Hampton does anyway. If fine sampling pH, you can mix various ratios of pH 3 and 6.5 buffers. The pH won't be linear with

[ccp4bb] preparation of citrate buffer pH3-6.5

2014-01-29 Thread sreetama das
Dear All,            We have obtained many tiny protein crystals in a condition containing 0.1M citric acid pH 3.5, 2M ammonium sulfate. The crystals are too small for mounting in loops.            We intend to vary the salt concentration & pH to obtain larger crystals.            Could anyon