Hi Peter,
we had a four similar cases recently. What really helped us was to add urea in
amounts of 1-2 M. We tested the possible influence on secondary structure by
limited proteolysis and CD/Trp-fluorescence and it seemed the protein
maintained the same overall conformation as in the absence
Hello all
I apolozize for a off topic question on BB.
I am working with small proetin-protein complex, each have molecular weight
10kDa. I elute this N-terminal His-tagged complex through Ni-NTA resin in
50mM of NaH2Po4, 0.3M of NaCl, 5% glycerol and 250mM of Imidazole.Similarly,
Lysis buffer and
