[CCP4BB@JISCMAIL.AC.UK] on behalf of Fernandez, Elias
J [efern...@utk.edu]
Sent: Friday, September 13, 2013 7:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] over-expression strategies
Yes, the gene's been codon-optimized. The predicted mRNA structure appears
stronger in the optimized ve
Elias Fernandez
[efern...@utk.edu]
Sent: Thursday, September 12, 2013 10:22 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] over-expression strategies
Dear CCP4ers,
We’ve been struggling with little (nearly none) expression of our protein, in
both E coli and with in vitro transcription/transl
Dear CCP4ers,
We've been struggling with little (nearly none) expression of our protein,
in both E coli and with in vitro transcription/translation methods. It
appears that our mRNA has high 2' structure with a low dG (theoretically
~760kcal/mol). If this is indeed the source of our problem, are t
Is your gene of interest smaller than 750bp ?
Then I would synthesize the gene with IDT and optimize it for E.coli that's 139$
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research In
