Re: [ccp4bb] optimizing ncs masks in DM + interpreting output

2009-11-10 Thread Klaus Fütterer
Francis, I still like to use Gerard Kleywegt's programme IMP (of the RAVE suite) to optimise NCS matrices before density modification in DM or other programs. Initial correlation coefficients (prior to solvent flattening) should be 20% or better. Below, the matrices are doubtful and densi

[ccp4bb] optimizing ncs masks in DM + interpreting output

2009-11-10 Thread Francis E Reyes
Hi all I have a rotation + translation component for the 2-fold given to me by GETAX using low resolution low quality experimental maps. I understand that these are input into DM for NCS averaging. What are signs of progress as well as how does one usually optimize the mask? When doing a