frustrating problem.
Mike Thompson
- Original Message -
From: "joybeiyang"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, September 7, 2010 12:30:04 PM GMT -08:00 US/Canada Pacific
Subject: [ccp4bb] off topic-aggregation of proteins
Hi everyone, sorry for this none CCP4 question, but I am wor
Dear Bei,
You question is not off topic, no apology needed.
Have you considered other possibilities than using detergents?
A little bit of glycerol might help, or e.g. a different salt, pH, buffer,
some additive, or simply salt concentration.
I once met a protein which was not soluble much beyon
Hi everyone, sorry for this none CCP4 question, but I am working on some tough
proteins which easily got aggregated after purification, I am thinking of using
some detergents rightnow, however, I am a newbie in this field, could you
please give me some advice on the usage of detergents?
here is
