, June 28, 2012 1:42 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] off topic: protein peptide interaction using DSF
Yes
add peptide in different concentrations to your protein and run the
assay*
Yes, or Sypro Orange
*Rule of
Yes
add peptide in different concentrations to your protein and run the assay*
Yes, or Sypro Orange
*Rule of thumb 20 kDa protein use 1 mg/ml 50 kDa protein use 0.5 mg/ml
also have a peptide which should not interact with your protein as control
Jürgen
On Jun 28, 2012, at 7:34 AM, rashmi panigra
Hi all,
Has anyone performed protein peptide interaction experiments using
flurophore on a real time PCR machine?
Please advice me on how the experiment is performed. Can I use ANS?
with regards
--
rashmi
