What if your mild urea condition unfolds your protein from a globular to a
stretched elongated form ? You probably have done CD already to verify that
this is not the case.
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Mol
Dear Careina,
Could you tell what reason you have to believe that your protein is
oligomerising? If your protein is forming oligomers, you should be able
to detect them using crosslinking or Native PAGE.
Do you have access to an analytical ultracentrifuge?
cheers
Ganesh
Le 14/11/12 08
Anyone have any advice on how to detect whether my protein is forming
oligomers? It is monomeric in the native state but I have reason to believe
that it may be oligomerising in mild concentrations of urea (intermediate
state).
I have tried cross linking and BN PAGE and they are inconclusive. SE
