r the gel and spend the 1-5 hrs there,
your initial conditions are lost anyways.
Zhijie
From: Careina Edgooms
Sent: Thursday, March 28, 2013 3:03 AM
To: Zhijie Li
Subject: Re: [ccp4bb] off topic, BN PAGE
Hi Zhijie
Thanks for the helpful reply. The attractive thing about BN PAGE (if it work
tical
ultracentrifugation, BiaCore, ITC and so on. I had a case in which I could
confidently show complex formation with gel-filtration and Biacore, but not
with BN gel.
Zhijie
From: Careina Edgooms
Sent: Wednesday, March 27, 2013 5:24 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] off topi
Hi
Has anyone found the coomassie in a BN PAGE to be interfering with the
oligomeric structure of their protein? If so, how did you deal with this?
Thanks
Careina
