CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] off-topic replay: glycerol in protein stock solutions
>
> Hi guys:
> I know this has been asked before, but I want to get (current) opinions and
> observations once again. Every once in a while, you work with a protein that
> needs to have
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Brett,
Thomas
Sent: Friday, March 04, 2011 8:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] off-topic replay: glycerol in protein stock solutions
Hi guys:
I know this has been asked before, but I
hello Bert
u can also add 50mM to 100 mM Argine and glutamate either alone or
combined together..it really works fine..although it will increase the
protein stability many fold but i dont know whether it will impede
crystallization or not..
others comments are welcome..
Faisal
SLS, JNU
New Delhi
Hi Tom:
Glycerol is fine in crystallization trials. As you say it may increase
your solubility, in your case that appears to be a good thing, for other
proteins, it makes them too soluble and then one may have to increase
the concentration to something more than the typical values.
Glycero
Hi Tom,
Adding glycerol to (crystallization) buffers is a very common practice when
working with membrane proteins. Many membrane proteins have been crystallized
(perhaps even the majority) with glycerol, even up to 30% v/v. So, at least for
membrane proteins, there is no problem.
Bert
On 3/
Hi guys:
I know this has been asked before, but I want to get (current) opinions and
observations once again. Every once in a while, you work with a protein that
needs to have a little something added to the buffer to keep it soluble. The
most common trick is addition of glycerol (usually 5-10%)
