Plus check the insoluble part or the whole cell.
Date: Sat, 5 May 2012 16:30:06 -0400
From: jubo...@jhsph.edu
Subject: Re: [ccp4bb] no expression
To: CCP4BB@JISCMAIL.AC.UK
Some very stupid questions from my side:- it is an expression vector and you
are in frame with your gene of interest
Some very stupid questions from my side:
- it is an expression vector and you are in frame with your gene of interest ?
- you are using E. coli strains suitable for expression ?
- have you tried adding glucose to the media to avoid leaky expression in case
your protein is toxic to E. coli ?
- the
Dear friends,
I am sorry for off-topic though it may be related indirectly!
I mutated a 60kDa protein (changing from X-->Pro). After doing all the steps,
I sequenced the expression vector. It seems everything is fine, even with
promoter, but, I can't express it. I tried different Tempratures,
