Re: [ccp4bb] modelling of modified RNA

2019-04-02 Thread Paul Emsley
On 02/04/2019 14:28, Sam Tang wrote: Hello Hello. I have been refining a protein-RNA complex with Refmac. The RNA we used was tagged with a Cy label. I modelled the RNA in Coot using the RCrane add-on and manually built the Cy tag. However after Refmac (also via Coot) the linkage between C

[ccp4bb] modelling of modified RNA

2019-04-02 Thread Sam Tang
Hello I have been refining a protein-RNA complex with Refmac. The RNA we used was tagged with a Cy label. I modelled the RNA in Coot using the RCrane add-on and manually built the Cy tag. However after Refmac (also via Coot) the linkage between Cy and the RNA was always broken. I guess I must hav