k.jpg
Jim
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Ed Pozharski
[epozh...@umaryland.edu]
Sent: Friday, November 09, 2012 8:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] model protein for ligand soaking
On Fri, 2012-11-09 at
Hi Uli,
I think Proteinase K and PMSF should work, though I never tried.
Cheers,
Dmitry
On 2012-11-09, at 9:12 AM, Ulrich Zander wrote:
> Hi All,
>
>
> For a tutorial, we would like to demonstrate the method of ligand soaking in
> protein crystals. I am thinking about using some sort of na
On Fri, 2012-11-09 at 15:12 +0100, Ulrich Zander wrote:
> Does anybody have a suggestion for a protein/ligand combination that
> could be used for that and that is commercially available?
Perhaps lysozyme complexed with some sugar?
--
Bullseye! Excellent shot, Maurice.
Human carbonic anhydrase II and any sulfonamide. We use a variety of
alkylsulfonamides (everyone can have a different ligand and they are
easy to model) or you can use commercially available inhibitors like
benzenesulfonamide, sulfanilamide, acetazolamide, etc. A 30 second soak
is sufficient to
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Dear Uli,
the geometric ligands (I3C, B3C, B4C) by Tobias Beck
(http://www.protein.ethz.ch/people/tobias/) are both commercially
available and easily identified. I3C is suitable for inhouse data
collection and provide a strong anomalous signal.
Of co
Hi All,
For a tutorial, we would like to demonstrate the method of ligand
soaking in protein crystals. I am thinking about using some sort of
native ligand or inhibitor that can be easily identified in the electron
density rather than halide or heavy atom derivatization.
Does anybody have a
