...@gmail.com]
> Sent: Wednesday, October 23, 2013 11:22 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] membrane protein optimization
>
> Hi CCP4BB Forks,
>
> In recently I got a membrane protein crystal in the quite normal
> membrane protein crystallization conditions as othe
d luck
toufic el arnaout
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of crystalboy
[yyb...@gmail.com]
Sent: Wednesday, October 23, 2013 11:22 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] membrane protein optimization
Hi CCP4BB Forks,
In recently I got a membra
Hi Frank,
Do not forget that membrane protein crystals are often fragile and
difficult to manipulate.
Finding good cryo condition can be difficult and small temperature
variation can destroy crystals
within minutes, this makes room temperature diffraction tests not always
obvious. The time
Hi Frank,
unfortunately it is very common with membrane protein crystals to get "stuck"
with diffraction quality around 20A.
From what you describe you could consider the following:
a) revise the detergent you solubilize your MP with (e.g. use OG instead of
DDM), and consider to change to anot
crystalboy
[yyb...@gmail.com]
Sent: Wednesday, October 23, 2013 5:22 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] membrane protein optimization
Hi CCP4BB Forks,
In recently I got a membrane protein crystal in the quite normal
membrane protein crystallization conditions as other persons reported,
l
Dear Frank,
It is difficult to say something without the diffraction images but probably
they are detergent ( or lipid) crystals. These crystals are in general "soft"
and don't diffract well.
My suggestion is to know well what your crystals really are before any
optimization.
Best,
Isabel
