Concerning "low resolution SAD phasing" I would like to add some information
here:
1. OASIS (version 2000) helped solving the unknown structure 1u9s in phasing
the 2.9A SAD data (
http://www.sciencemag.org/content/suppl/2004/09/30/306.5693.104.DC1/Krasilnikov.SOM.pdf
).
2. OASIS-2004 helped solvi
Tommi,
Since you ask, SHELX and other 'direct methods' run out of steam at about
1.2A and for many years very few unknown structures have been solved at
lower resolution than 1.2A. Recntly this picture was changed by the
introduction of ARCIMBOLDO, which has solved a number of structures ab
init
Hi,
Thanks for all the comments, i was more wondering what the state of
the art might
be here, we have done similar thing at 4 Å (with 3 Å data though) 10
years or so back
with SnB. Maybe we need to get a heavier atom derivative indeed the
break the phases,
but i'll keep banging for now..
http://www.rsc.org/shop/books/2008/9780854042722.asp
Original message
>Date: Wed, 15 Jun 2011 16:36:59 +0300
>From: CCP4 bulletin board (on behalf of tommi kajander
>)
>Subject: [ccp4bb] low res. SAD phasing
>To: CCP4BB@JISCMAIL.AC.UK
>
>Dear all,
>
>Does
Hi Tommi,
Give it a try?
You give very little information, e.g.
- the data quality,
- possible radiation damage,
- isomorphism with a native data set, and
- what you have tried so far.
If you have a native data set with an isomorphous unit cell, try SIRAS instead
of SAD.
Tim
On Wed, Jun 15,
Dear all,
Does anyone have suggestions for 6 Å resolution phasing with large
number (40-50) Se sites (SAD so far)??
Thanks a bunch,
Tommi
Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. B
