appreciated.
Thanks
SDY
Date: Thu, 8 Nov 2012 14:36:59 +0200
From: mbfro...@post.tau.ac.il
Subject: Re: [ccp4bb] low-resolution and zinc
To: CCP4BB@JISCMAIL.AC.UK
The experiment should be very problematic if I can't determin
I am using Refmac_5.7.0029 in CCP4ThanksSDY Date: Fri, 9 Nov 2012 20:35:48
+0200
From: mbfro...@post.tau.ac.il
Subject: Re: [ccp4bb] low-resolution and zinc
To: CCP4BB@JISCMAIL.AC.UK
What program do you use for refinement?FF
Dr Felix Frolow
Professor of Structural Biology and
ow what is missing, I also attached the log file which generated the
> ZN-His coordination.
>
> Any help is highly appreciated.
>
> Thanks
> SDY
>
> Date: Thu, 8 Nov 2012 14:36:59 +0200
> From: mbfro...@post.tau.ac.il
> Subject: Re: [ccp4bb] low-resolution and zinc
> To:
various
>> possibilities.
>>
>> Herman
>>
>> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Felix
>> Frolow
>> Sent: Thursday, November 08, 2012 10:36 AM
>> To: CCP4BB@JISCMAIL.AC.UK
>> Subject: Re: [ccp4bb] low-resolution
] On
Behalf Of Felix Frolow
Sent: Thursday, November 08, 2012 1:14 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] low-resolution and zinc
I do not see with what you do not agree in what was written
(maybe not very carefully). One determine sp
oups manually, but one has to test the various
> possibilities.
>
> Herman
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Felix
> Frolow
> Sent: Thursday, November 08, 2012 10:36 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] l
Yogi
From: mbfro...@post.tau.ac.il
Subject: Re: [ccp4bb] low-resolution and zinc
Date: Wed, 7 Nov 2012 23:35:35 +0200
To: ccp4...@hotmail.com
me and help which I was very much in need of.
>
> Thanks
> Yogi
>
> From: mbfro...@post.tau.ac.il
> Subject: Re: [ccp4bb] low-resolution and zinc
> Date: Wed, 7 Nov 2012 23:35:35 +0200
> To: ccp4...@hotmail.com
>
> It is THE BOOK published in 1976! There is a chapter
duction media.
>>
>> I am trying to search the refence in google. Are you refering to the Book
>> published in 1976 titled "protein crystallography", if not could you please
>> kindly direct me to right reference.
>>
>> I sincerely appreciate your time and
direct me to right reference.
>
> I sincerely appreciate your time and suggestion.
>
> Warm reagrds,
> SDY
>
>
>
>
>
>
>
> --
> From: mbfro...@post.tau.ac.il
> Subject: Re: [ccp4bb] low-resolution and zinc
> Date: Wed, 7 Nov 201
; kindly direct me to right reference.
>
> I sincerely appreciate your time and suggestion.
>
> Warm reagrds,
> SDY
>
>
>
>
>
>
>
>
> From: mbfro...@post.tau.ac.il
> Subject: Re: [ccp4bb] low-resolution and zinc
> Date: Wed, 7 Nov 2012 19:35:21 +0
Tryptone and/or yeast extract is loaded with metals, including zinc. It
is almost impossible to keep zinc contamination at bay without using
carefully defined media and taking heroic measures. (I know this because
of the difficulty of making overexpressed metallo-substituted
zinc-enzymes when t
On Wed, 2012-11-07 at 11:29 -0600, SD Y wrote:
> https://www.dropbox.com/s/4jd6gdor87ab9lj/Zn-coordination.png
Your sigma level of 6.5 seems a bit low, so maybe it is a different
metal. But on your main question - yes, metal binding proteins do pick
up metals from the media. Once metal is coord
Dear all,
I have a related question to the one I have posted "low resolution and SG", on
which I am still working based on the suggestions I have got.
The model I have used, has Zn co-ordinated well in tetrahydral fashion by 3 cys
and 1 His residues. They have add Zn in to their experiment
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