Do you have an inactive variant of the protein? If yes, use limited
proteolysis in the presence of methylated histones to find out which
flexible parts are really important and or protected by complex
formation. Further, try to crystallize a complex of inactive demethylase
and methylated histon
From: CCP4 bulletin board on behalf of dongxiaofei
<18811070...@163.com>
Sent: 04 July 2017 16:22:39
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] long loop
Dear ALL,
I want to make a protein crystal,but there is a long loop between domains of
protein , which contains two small domains
Fab fragment binding towards long loop
在2017年07月04日 23:22，dongxiaofei 写道：
Dear ALL,
I want to make a protein crystal,but there is a long loop between domains of
protein , which contains two small domains owning about 40 amino acids
respectively and a loop about 70 amino acids.
Loop is so lo
Dear ALL,
I want to make a protein crystal,but there is a long loop between domains of
protein , which contains two small domains owning about 40 amino acids
respectively and a loop about 70 amino acids.
Loop is so long and flexible ,but I don't want to delete some fragments,because
it may be i
