Gloria,
Here is how we are now doing it, courtesy of Yu-Jing's and Merlin's fine
development work. We have also tried it to quantitatively remove tags by TEV
cleavage, but we haven't solved that problem yet.
Regards,
Michael
Tan, Yu-Jing, Wei-Han Wang, Yi Zheng, Jinlan Dong, Giovanni Stefano
We are trying to apply this approach for the first time to define a
crystallizable domain of our protein of interest. Can anyone send a
protocol that includes exactly how to do the mass spectrometry
measurement? Our core lab here doesn't know and I'm just not that gifted.
Happy October, G
