Dear All,
As we also work with phages in the lab, we unfortunately have some experience
with this (although the phages we work with haven't been identified in our
cultures).
I second Tassos suggestion to close down normal operations for a week or more
and clean with the whole lab involved and
We have suffered from that a lot at the NKI a few years back.
What worked for us was a combination of strategies, that were all mentioned
before but I will repeat:
1. Buy T1 phage-resistant strains, and also use an old incubator (and luckily
an old building
that was available at the time) to 'e
Dear Opher,
It was also our idea how to get over it since several years ago. We
did it based on a philosophy that any bacteria infected with a phage
will not be infected by the same phage again. Therefore, we let our
BL21/DE3 strain infected in the lab and when most were died, some
cells
Hi,
We had a massive phage infection at the SGC in 2004; all our washing and
sterilization efforts could only solve the problem temporarily. I then
recovered a phage-resistant subclone of BL21(DE3), and prepared derivative
strains with pRARE2 (the tRNA plasmid in Rosetta2) or other plasmids. We
Dear Aidong,
Some thoughts...
Are you sure the problem is actually phage-related? I ask because you are still
having problems using phage-resistant stains...
Acid-washing your glassware, and a long-dry autoclave sterilisation (as
previously suggested) should really do the trick. A good *manu
Dear Aidong,
we never had that problem but I heard that only long dry(!) heat
treatment of glassware destroys the phages.
No need to say that you have to do it with ALL your glassware.
Good luck,
Matthias
-
Dr. Matthias Zebisch
Division of Structural B
Many thanks for your rapid inputs.
We used to make glyerol stocks but now we do not since the protein
expression is not stable. Our lab is about 10 students, who have to
make their own proteins so our three large-scale shakers are very
crowded every day. We did try a formaldehyde gas trea
