Re: [ccp4bb] ion exchange chromatography problem

...forgot to mention I used Q sepherose from Sigma for the IEC

[ccp4bb] ion exchange chromatography problem

Hello everyone, I have a question about ion exchange chromatography. - I have a membrane protein (pI 9.5), 13 KDa - It's fused to MBP (maltose binding protein pI 5.1, 44 KDa) to prevent inclusion body formation during high level expresscion. - The final protein has a pI of 6.1, 57 KDa --> The p