Since water is unbuffered, you are likely changing the pH of the solvent,
desorbing additional protein. If you are working unbuffered, your IEX
results will be unpredictable.
Roger Rowlett
On Sep 29, 2011 4:55 AM, "K Singh" wrote:
> Dear All,
> Apologies for bit off-topic. I am in process of puri
Dear All,
Apologies for bit off-topic. I am in process of purifying a protein by
ion-exchange (cation)
using 0.5-3M NaCl gradient. And I get a sharp peak which is western
positive. Following elution,
I wash the column >10 column volume with 3M NaCl. and now when I
wash the column
with water, I ge
