Thanks to all that replied. You might find this useful.
The question was: How to select an insect expression system for
mammalian/viral glycoproteins? The following is a summary of the replies.
*1. Baculovirus system*
1) For most proteins, the level of expression is far greater with
bacuovir
865-287547
Original message
Date: Wed, 28 May 2008 10:40:16 -0400
From: Yong-Fu Li <[EMAIL PROTECTED]>
Subject: [ccp4bb] insect expression system
To: CCP4BB@JISCMAIL.AC.UK
Hi,
I searched my mail box for possible answers you have
given but couldn't find any. The questio
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Original message
>Date: Wed, 28 May 2008 10:40:16 -0400
>From: Yong-Fu Li <[EMAIL PROTECTED]>
>Subject: [ccp4bb] insect expression system
>To: CCP4BB@JISCMAIL.AC.UK
>
> Hi,
>
>
Hi,
It is called Bacmam. Please see the following reference, it is not
the land mark reference, if you dig pubmed you should be able to find
the first description of the system.
BacMam recombinant baculovirus in transporter expression: a study of
BCRP and OATP1B1.
Protein Expr Purif. 2006 Jun;47(
I searched my mail box for possible answers you have given but couldn't
find any. The question is about selecting insect expression systems for
mammalian or viral glycosylated proteins. There is confirmed expression of
the target proteins in mammalian cells. They are secreted into the media.
Hi, with S2 you can get away without figuring out if the virus works, then
again you probably need a stable cell line... (so which ever works...?)
(OBS! cant do SeMet labelling with S2!! or if someone can please tell me)
-Tommi
Quoting Yong-Fu Li <[EMAIL PROTECTED]>:
> Hi,
>
> I searched my ma
Hi,
I searched my mail box for possible answers you have given but couldn't find
any. The question is about selecting insect expression systems for mammalian
or viral glycosylated proteins. There is confirmed expression of the target
proteins in mammalian cells. They are secreted into the media.
