Thank you all for your suggestions, I copied them below
I also found a paper for making Src and Abl catalytic domains Protein
Science Dec 2005
The kinase domain of FAK can be made constitutively active by a mutation
and expressed in E. coli. It's called "Super FAK Kinase". But i am afraid
it is rather specific. If you don't get a better lead I can send you the
sequence and a reference.
Gloria Borgstahl wrote:
Does anyone know of a good
Does anyone know of a good way to phosphorylate the Tyr on a protein for
structural studies? Is there a generic kinase that can be coexpressed or
purified for phosphorylation?
The pCMF Amber codon system is very expensive
and Glu really doesn't mimic pTyr all that well.
Any ideas/help would be a
