Re: [ccp4bb] how to deal 3 twin-related domains

2013-05-08 Thread Andrea Thorn
Dear Fulvio, what did your L-Test with the unmerged (P1) data look like? In order to use twin refinement you should be sure that the structure is twinned. Did you try all possible subgroups of R3? Generally, a triplet can be treated as any other pseudo-merohedral twin, given it really is a trip

[ccp4bb] how to deal 3 twin-related domains

2013-05-07 Thread Fulvio Saccoccia
Dear ccp4 users, I have a reflections file obtained from a crystal initially scaled in H3. All the attempts to phase by Molecular Replacement were not successful and I succeeded only in P1. Based on previously solved structures from the same protein, I suspected that twinning affected crys