Re: [ccp4bb] high pI protein

2008-07-09 Thread Das, Debanu
ent: Wednesday, July 09, 2008 3:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] high pI protein Hi all, We have a protein with high pI and we can only dissolve it at pH 10.2. Is this a reasonable pH to work with? Should we try to bind something to it to decrease the pH we are working with? We wo

[ccp4bb] high pI protein

2008-07-09 Thread Michael Colaneri
Hi all, We have a protein with high pI and we can only dissolve it at pH 10.2. Is this a reasonable pH to work with? Should we try to bind something to it to decrease the pH we are working with? We would appreciate all suggestions. Mike Colaneri