re in E.coli.
Good luck,
Mike Thompson
- Original Message -
From: "Artem Evdokimov"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Saturday, January 1, 2011 6:56:03 AM GMT -08:00 US/Canada Pacific
Subject: Re: [ccp4bb] help with kinase purification
Each protein is different, but t
Each protein is different, but there are indeed similarities within classes.
It can be dangerous/unproductive to generalize -- you will find out
experimentally whether you can assume things or not.
Depending on the expression method and the design of your construct you may
or may not have a tag. H
Hi All,
A very happy new year to everyone. I am beginning to purify a
novel kinase and was wondering if there's a standard protocol / method
that can be utilized to begin purification of a kinase. I am new to
the field of kinases and would appreciate any help with respect to the
pre
