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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stephen
Cusack
Sent: Monday, October 09, 2017 5:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] help identifying unknown density
Dear All,
I am refining the crystal structure of an E. coli expressed protein at
://www.normandcyr.com
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Stephen Cusack
[cus...@embl.fr]
Envoyé : 9 octobre 2017 11:40
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] help identifying unknown density
Dear All,
I am refining the crystal
You may have crystallized an enzyme that uses a phosphoramidate intermediate.
https://en.wikipedia.org/wiki/Phosphoramidate
On Oct 9, 2017, at 10:40 AM, Stephen Cusack
mailto:cus...@embl.fr>> wrote:
Dear All,
I am refining the crystal structure of an E. coli expressed protein at 2.65
A re
University of Pavia
Via Ferrata, 9
I-27100 Pavia - ITALY
http://fornerislab.unipv.it
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stephen
Cusack
Sent: lunedì 9 ottobre 2017 17:40
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] help identifying
40 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] help identifying unknown density
>
> Dear All,
>
> I am refining the crystal structure of an E. coli expressed protein at
> 2.65 A resolution. The crystals grew in
>
> 0.1 M MES pH6, 0.7 M sodium formate pH 6. Ni-NTA
Could it be a formate ion (HCO2−)? _Gang
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stephen
Cusack
Sent: Monday, October 09, 2017 5:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] help identifying unknown density
Dear All,
I am