Re: [ccp4bb] help identifying unknown density

2017-10-10 Thread Jiyong Su
- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stephen Cusack Sent: Monday, October 09, 2017 5:40 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] help identifying unknown density Dear All, I am refining the crystal structure of an E. coli expressed protein at

[ccp4bb] RE : [ccp4bb] help identifying unknown density

2017-10-10 Thread Cyr Normand
://www.normandcyr.com De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Stephen Cusack [cus...@embl.fr] Envoyé : 9 octobre 2017 11:40 À : CCP4BB@JISCMAIL.AC.UK Objet : [ccp4bb] help identifying unknown density Dear All, I am refining the crystal

Re: [ccp4bb] help identifying unknown density

2017-10-10 Thread CRAIG A BINGMAN
You may have crystallized an enzyme that uses a phosphoramidate intermediate. https://en.wikipedia.org/wiki/Phosphoramidate On Oct 9, 2017, at 10:40 AM, Stephen Cusack mailto:cus...@embl.fr>> wrote: Dear All, I am refining the crystal structure of an E. coli expressed protein at 2.65 A re

Re: [ccp4bb] help identifying unknown density

2017-10-09 Thread Federico Forneris
University of Pavia Via Ferrata, 9 I-27100 Pavia - ITALY http://fornerislab.unipv.it -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stephen Cusack Sent: lunedì 9 ottobre 2017 17:40 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] help identifying

Re: [ccp4bb] help identifying unknown density

2017-10-09 Thread Eleanor Dodson
40 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] help identifying unknown density > > Dear All, > > I am refining the crystal structure of an E. coli expressed protein at > 2.65 A resolution. The crystals grew in > > 0.1 M MES pH6, 0.7 M sodium formate pH 6. Ni-NTA

Re: [ccp4bb] help identifying unknown density

2017-10-09 Thread Gang Dong
Could it be a formate ion (HCO2−)? _Gang -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stephen Cusack Sent: Monday, October 09, 2017 5:40 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] help identifying unknown density Dear All, I am