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http://www.rsc.org/shop/books/2008/9780854042722.asp
Original message
>Date: Mon, 8 Aug 2011 15:26:43 -0700
>From: CCP4 bulletin board (on behalf of Paul Kraft
>)
>Subject: Re: [ccp4bb] gst-tag protein purify probl
.
--- On Sat, 8/6/11, Carlos Kikuti wrote:
From: Carlos Kikuti
Subject: Re: [ccp4bb] gst-tag protein purify problem
To: CCP4BB@JISCMAIL.AC.UK
Date: Saturday, August 6, 2011, 5:13 PM
What happens if you load your elution fraction into a size exclusion column? If
your protein of interest comes in
us by e-mail
> is taken to accept the risks in doing so.
> From: LISA
> To: CCP4BB@JISCMAIL.AC.UK
> Sent: Thursday, August 4, 2011 11:51 AM
> Subject: [ccp4bb] gst-tag protein purify problem
>
> hi guys,
> I have a DNA binding protein and expressed the DNA binding domain (1
doing so.
From: LISA
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thursday, August 4, 2011 11:51 AM
Subject: [ccp4bb] gst-tag protein purify problem
hi guys,
I have a DNA binding protein and expressed the DNA binding domain (150 aa) with
his-sumo tag or gst tag at the n-terminal. I tried to purified it
hi guys,
I have a DNA binding protein and expressed the DNA binding domain (150
aa) with his-sumo tag or gst tag at the n-terminal. I tried to purified it
with Ni column or Gst column separately. But purity is lower than 50% after
Ni or GST column. This protein only stable with 1M Nacl or higher. I
