Re: [ccp4bb] generate symmetry mates of EM structures

2021-05-21 Thread Xiao Lei
Hi, Stefano, Thanks. I use Pymol 2.4. I figured out that if I downloaded the PDB (5L93) RCSB website (biological assembly 1 PDB file), Pymol would show each state of the structure (3 chains) individually, if I execute the "set all_states, on" command, I can see the assembly. Best, Xiao On Fri,

Re: [ccp4bb] generate symmetry mates of EM structures

2021-05-21 Thread Xiao Lei
Dear David, Thank you for your help. It seems the problem is due to the Pymol program. I could only see 3 chains in Pymol from the assembly PDB downloaded from the RCSB website (biological assembly 1 PDB file). If I use UCSF Chimera, I can see 18 chains. With others' help, I can also use "sym" com

Re: [ccp4bb] generate symmetry mates of EM structures

2021-05-21 Thread David Armstrong
Dear Xiao, For public entries in the PDB archive, it is possible to download the coordinates of the assemblies defined in the PDB entry. If you visit the page for the entry at PDBe (pdbe.org/5l93) and select 'Downloads' you have the option to download various data, including mmCIF files cont

[ccp4bb] generate symmetry mates of EM structures

2021-05-21 Thread Xiao Lei
Dear Community, I wonder if there is a way to generate symmetry mates of EM structures? For example for PDB code 5L93, I tried PISA assembly server and Pymol to generate assembly (18 chains) but failed because it is not a crystal structure. I tried to download assembly directly from RCSB databank