ong Yu
> [nkyuz...@gmail.com]
> Sent: Thursday, November 07, 2013 2:53 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] few questions about resolving new structure through
> MR
>
> Thanks Francis,
>
> No, only one molecule in the asu. The Matthews Coefficient i
anu.
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Zhihong Yu
[nkyuz...@gmail.com]
Sent: Thursday, November 07, 2013 2:53 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] few questions about resolving new structure through MR
Thanks Francis,
No, only one molecule in the
Thanks Francis,
No, only one molecule in the asu. The Matthews Coefficient is 3.3,
corresponding solvent content is 62.6%, maybe that's why this crystal show
such weak diffraction?
Zhihong
On Thu, Nov 7, 2013 at 5:37 PM, Francis Reyes wrote:
>
> Do you expect more than one molecule in the asym
Do you expect more than one molecule in the asymmetric unit?
Determined from the Matthews Coefficient (poor), size exclusion column
(better), or self RF (best) ?
On Nov 7, 2013, at 8:36 AM, Zhihong Yu wrote:
> Hi, all
>
> I'm a rookie in resolving a brand new structure. I have some question
gt;
>>
Greg Costakes, Ph.D.
>> Department of Structural Biology Purdue University Hockmeyer
>> Hall, Room 320 240 S. Martin Jischke Drive, West Lafayette, IN
>> 47907
>>
>>
>> --------------------
>>
>>
>>
>>
- -
---
> *From: *"Zhihong Yu"
> *To: *CCP4BB@JISCMAIL.AC.UK
> *Sent: *Thursday, November 7, 2013 11:36:51 AM
> *Subject: *[ccp4bb] few questions about resolving new structure through MR
>
>
> Hi, all
>
> I'm a rookie in resolving a brand new structure. I
907
- Original Message -
From: "Zhihong Yu"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thursday, November 7, 2013 11:36:51 AM
Subject: [ccp4bb] few questions about resolving new structure through MR
Hi, all
I'm a rookie in resolving a brand new structure. I have some questions for my
Count yourself lucky that you may have a partial solution for your
structure with only 30% identity. The question now is: can you see any
reasonable, traceable electron density for domain A?
Cheers,
___
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Depar
Hi, all
I'm a rookie in resolving a brand new structure. I have some questions for
my current case and look forward to some suggestions.
Now I’m working on a protein like this:
N-ter(55aa)—domainA(110aa)—linker(30aa)—domainB(150aa)—C-ter(20aa), I got a
diffraction data just to 3.5Å, and there is
