When we develop our gel we recently keep getting a horizontal line of
stain at 5 mol weight in all lanes of our gel. (This is not a
feature of interest except that the protein that we are interested happens
to be 5 mol weight). I would appreciate any ideas on how we can get
rid of
Hi Mike
Sometimes keratin from your skin (fingers, nails, etc) appear in the gels
as a band around 48-52 kDa. Take care in the handling of the
electrophoresis should be a solution.
Other possible reason could be a contamination of the sample loading
buffer, also with keratin or with other protein
Hello,
When we develop our gel we recently keep getting a horizontal line of stain
at 5 mol weight in all lanes of our gel. (This is not a feature of
interest except that the protein that we are interested happens to be 5
mol weight). I would appreciate any ideas on how we can get rid o
