Both will probably work.
Expression in insect cells is most likely to work well, but will of course
be more expensive and time consuming. There's also some slight possibility
of getting unwanted glycosylation - which is not necessarily a problem.
I've had good success expressing VHH fragments (s
Dear all,
I'd like to express a single chain variable fragment (scFv) for
crystallization purposes, but cannot decide whether I should do it in
insect cells (let the antibody be secreted into culture medium) or
bacteria (let the antibody be secreted into periplasmic space).
If someone could give
