bject: [ccp4bb] estimate protein in Incl bod
We have our recombinant protein expressed in inclusion bodies we just wash
the IBs and perform solubilization and refolding. Our solubilisation
protocol
mentions making up the volume to contain 7-8 mg / ml protein.
1. how can we determine the total pr
We have our recombinant protein expressed in inclusion bodies we just wash
the IBs and perform solubilization and refolding. Our solubilisation protocol
mentions making up the volume to contain 7-8 mg / ml protein.
1. how can we determine the total protein content in the inclusion bodies
itself
