Re: [ccp4bb] effect of high salt on protein

2007-04-07 Thread Artem Evdokimov
7;re worried. Artem -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of bputcha Sent: Saturday, April 07, 2007 7:19 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] effect of high salt on protein Hi all, I keep my protein at 1 M NaCl, at some stage of purifi

[ccp4bb] effect of high salt on protein

2007-04-07 Thread bputcha
Hi all, I keep my protein at 1 M NaCl, at some stage of purification, for about 30 minutes. Protein does not precipitate out at that stage and susequently when the salt concentration is reduced. I would like to know if such a high salt concentration can perturb ( irreversibly) the structure or