: Saturday, March 08, 2014 7:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] density near Histidine
Hi,
During refinement, I see a strong density next to His and I have tried metal
ions and water but it seems to be different. Known components of
crystallization are Tris, NaCl, BME, MgOAc
Hi SDY,
It would be unlikely unless you have other reasons to believe it (biology,
surrounding environment), but that blob looks a little like a possible
phospho-histidine. It might be worth a looking into?
That said, buffer or metals are probably the more likely explanation.
Shane Caldwell
McGi
Hello,
it is not the first time that I see such electron densities near to His
residue.
However, in my structures (3N30, 3N32), I was sure that Zn, Pt or other
metals were present in the crystallization condition.
You have tried to refine with Zn rather than Mg that seems to be present
in the
Dear SDY,
It looks like ethylene glycol to me. Try EDO
(http://ligand-expo.rcsb.org/pyapps/ldHandler.py?formid=cc-index-search&operation=ccid&target=EDO)
Avinash
--
Avinash S. Punekar
Uppsala University
