han there would be without it (and no extra
>> diffraction strength), so that is another very good reason to avoid it, or
>> to buffer exchange it out before the diffraction experiment.
>>
>> Elspeth
>>
>> -Original Message-----
>> From: CCP4 bulletin b
l Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jim
Pflugrath
Sent: 23 November 2011 18:11
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] dark progression of radiation damage
Any cacodylate buffer will cause gas to be produced. One only needs a minute
exposure
CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jim
> Pflugrath
> Sent: 23 November 2011 18:11
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] dark progression of radiation damage
>
> Any cacodylate buffer will cause gas to be produced. One only needs a minute
&
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] dark progression of radiation damage
Any cacodylate buffer will cause gas to be produced. One only needs a minute
exposure on a modern home lab source to see this happening. I suggest that
everyone avoid cacodylate in their crystallization drops that end up
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Sanishvili,
Ruslan [rsanishv...@anl.gov]
Sent: Wednesday, November 23, 2011 11:49 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] dark progression of radiation damage
I think I need to clarify couple of things in my recent post
the crystal as large crystals are almost never homogeneous.
> Cheers,
> Nukri
>
>
>
>
> -Original Message-
> From: CCP4 bulletin board on behalf of James Holton
> Sent: Sun 11/20/2011 2:31 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] dark progression of
of the robot can worm up somewhat. Therefore, comparing diffraction before and after
storage is not always valid.
Also beware of comparing diffraction quality from different parts of the
crystal as large crystals are almost never homogeneous.
Cheers,
Nukri
-Original Message-
From:
tion quality from different parts of the
crystal as large crystals are almost never homogeneous.
Cheers,
Nukri
-Original Message-
From: CCP4 bulletin board on behalf of James Holton
Sent: Sun 11/20/2011 2:31 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] dark progression of radiation damage
Mark's comment below reminded me of a quandary that is starting to
develop in the rad dam field. The idea of the "free radical cascade"
continuing to damage protein crystals even after the beam has been
turned off seems to have originated on page 253 of Blundell and Johnson
(1976), and I thi
