On 12-01-24 08:39 AM, Regina Kettering wrote:
We have a Honeybee system but do not usually use
proteases. The biggest problem we have found is that if
anything precipitates in the tips they have to be washed
very well, usually wit
Hi Horacio,
we have a Cartesian Honeybee and perform trypsin-containing trials by setting
up drops with a dedicated protein+trypsin tip, which we then wash extensively
with water, 6 M guanidinium hydrochloride, then water, isopropanol and again
water.
hth,
ciao,
Sebastiano
On Jan 24, 2012, at
would also
alleviate the protease problem.
Regina
From: Horacio Botti
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, January 24, 2012 7:12 AM
Subject: [ccp4bb] Crystallization robot and trypsin
Dear all
We may use a Honeybee 963 robot to screen crystallization
Dear all
We may use a Honeybee 963 robot to screen crystallization conditions for
trypsin-containing protein samples and we are worried about robot
contamination by residual protease.
How do you normally clean robots when using this kind of sample? Your
suggestions/recommendations will be appr
Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034
Regd. England 2177994, VAT Reg. GB 480 7371 36
> -Original Message-
> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
> Nalam, Madhavi
> Sent: 09 January 2008 14:57
> To: CCP4BB@JISCMAIL.AC.UK
> Subjec
What Jan says (in brackets) is true and very important. If you go to
great lengths finding the best robot, you might as well use the best
plates, and the MRC plates are the best by far that I've ever used.
http://www.innovadyne.com/products_iplateSD2.html
Andreas
Jan Lowe wrote:
Since eve
Since everybody is showing off their kit (and we have been very well
supported by Innovadyne): we at the LMB in Cambridge are extremely happy
with our Innovadyne Screenmaker 96+8. It has been doing around 8000
plates per year for the past three years and it works well between 100
and 500 nl (us
We have been quite happy with our crystallization robot from Innovadyne. It
transfers from deep well blocks to crystallization plates, and very reliably
sets up 200nl + 200nl drops. We have tried smaller drops with decent
success, which works better with certain plates. It can be easily
programmed,
What a great time in crystallization; lots of options, lots of choices.
After reviewing the automation available for crystallization and our unique
needs we decided on the Phoenix. We have used the Phoenix from Art Robbins
Instruments with excellent results since installation October 2006.
Eac
CP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallization robot
Not to inject a commercial tone, but there seems to be a bias in the
posting below. The ceramic tips are breakable, but not all find them
incredibly so. And the price is less then $300, not $700.
-Original Message-
From:
I was wrong on the price- yes $300/per, but still, it was a lot of money
when we had catastrophic crash several years ago (due to something going
haywire- not a script or operator error). I know the nitonol tips are
not fool-proof- they just seem a bit more forgiving than the ceramic
tips.
As
half Of
> Lisa A Nagy
> Sent: Wednesday, January 09, 2008 5:21 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] crystallization robot
>
> We chose the Phoenix crystallization robot because:
>
> It has no expensive consumables (tips) intrinsic to the machine. This
>
] crystallization robot
We chose the Phoenix crystallization robot because:
It has no expensive consumables (tips) intrinsic to the machine. This
was also a big item for us because we worry about being able to run the
machine for more than 3 years. Would the tips for our 2008 machine be
available in 2014
Nagy
Sent: Wednesday, January 09, 2008 11:21 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallization robot
We chose the Phoenix crystallization robot because:
It has no expensive consumables (tips) intrinsic to the machine. This
was also a big item for us because we worry about being
rade
Parkville VIC 3052
Australia
tel: +61 (0)3 9662 7326
fax: +61 (0)3 9662 7101
email: [EMAIL PROTECTED]
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Lisa A Nagy
Sent: Thursday, 10 January 2008 4:49 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] c
] crystallization robot
Looking at the mosquito, it doesn’t have any cover-slip handling
robotics, either. So it’s the same thing- rearrange the dispense
location and flip the cover- which is either a glass plate or mylar
or a tape seal.
--
Lisa Nagy
University of Alabama-Birmingham
[EMAIL PROTECTED]
:56:34 -0500
>From: "Nalam, Madhavi" <[EMAIL PROTECTED]>
>Subject: [ccp4bb] crystallization robot
>To: CCP4BB@JISCMAIL.AC.UK
>
>Hi,
>Sorry for the non-ccp4 related question.
>We are planning to buy a crystallization robot. We looked at the
>'Mosquito'
deal.
I am certain that the mosquito sheets would work on any robot.
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Van Den Berg, Bert
Sent: Wednesday, January 09, 2008 11:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallization robot
The mosquito has
on behalf of Lisa A Nagy
Sent: Wed 1/9/2008 11:53 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallization robot
Looking at the mosquito, it doesn't have any cover-slip handling robotics,
either. So it's the same thing- rearrange the dispense location and flip the
cover
Looking at the mosquito, it doesn't have any cover-slip handling
robotics, either. So it's the same thing- rearrange the dispense
location and flip the cover- which is either a glass plate or mylar or
a tape seal.
--
Lisa Nagy
University of Alabama-Birmingham
[EMAIL PROTECTED]
It doesn't have a cover slip flipper.
We only do sitting drops with our robot- even large volumes (2 ul
drops).
We don't have the space to store the volume of linbro plates that would
be generated by a robot.
Also, 24 well linbro plates use a lot of well solution- making them very
expensive fo
AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallization robot
We chose the Phoenix crystallization robot because:
It has no expensive consumables (tips) intrinsic to the machine. This
was also a big item for us because we worry about being able to run the
machine for more than 3 years
We chose the Phoenix crystallization robot because:
It has no expensive consumables (tips) intrinsic to the machine. This
was also a big item for us because we worry about being able to run the
machine for more than 3 years. Would the tips for our 2008 machine be
available in 2014?
It is easy to
Douglas Oryx6 or 8. It works well on sitting drop and microbatch in our
division.
Nalam, Madhavi wrote:
Hi,
Sorry for the non-ccp4 related question.
We are planning to buy a crystallization robot. We looked at the
'Mosquito'. We felt it is good for setting 96 well plates (for screening
the condi
Hi,
Sorry for the non-ccp4 related question.
We are planning to buy a crystallization robot. We looked at the
'Mosquito'. We felt it is good for setting 96 well plates (for screening
the conditions). Though they say that we can use it for 24 well plates
(hanging drop) it didn't seem to be ideal bec
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