Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

We've been using the Mitegen MicroRT product for a while and it works well, but I'm anxious to try Filip Van Petegem's paratone oil method. I've used paratone as a cryo before, and spent a lot of time trying to wick away the liquid around the crystal - in the case of room-temp mounting the crystal

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

Dear Cedric, Another possible method for RT data collection is the new humidifier device HC1b developed at the EMBL that is now available for use at the ESRF (see links below). It works in a very similar way to the FMS but a little easier to use and can be used at the ESRF with very little ha

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

Dear Cedric, We use a much easier test for mounting crystals at room temperature: just coat the crystal with paratone oil and mount your crystal in a standard cryoloop. The oil will slow down evaporation enough - no special tools required. You don't even need to remove all the liquid as you woul

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

: Nobuhisa Watanabe To: CCP4BB@JISCMAIL.AC.UK Date: 01/16/2009 09:55 AM Subject: Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature If you just want to check crystals at room temperature, we have developed a "Direct Ex Plate" after Acta Cryst. D58

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

If you just want to check crystals at room temperature, we have developed a "Direct Ex Plate" after Acta Cryst. D58(10), 1527-1530 (2002). Please check, http://www.labo.co.jp/contents/direct_ex.html (Sorry for Japanese) Nobuhisa Watanabe === Synchrotron Radiation Research Center Department of Bio

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

From: CCP4 bulletin board on behalf of cedric bauvois Sent: Fri 1/16/2009 9:27 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature Dear CCP4ers, in their paper entitled " Using cryoloops for X-ray data collectio

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

cedric bauvois wrote: Dear CCP4ers, in their paper entitled " Using cryoloops for X-ray data collection from protein crystals at room temperature: A simple applicable method" ( *Journal of Crystal Growth* Volume 281, Issues 2-4

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

http://www.mitegen.com/ Cheers Carsten -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]on Behalf Of cedric bauvois Sent: Friday, January 16, 2009 9:27 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryoloops for X-ray data collection from protein

Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

Hi Cedric, I used these, but just for testing crystals. I was afraid that the crystal might move in the loop. For testing it worked pretty good. http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html There might be more suppliers. Please send a sum

[ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

Dear CCP4ers, in their paper entitled " Using cryoloops for X-ray data collection from protein crystals at room temperature: A simple applicable method" ( *Journal of Crystal Growth* Volume 281, Issues 2-4