Thanks to all for the suggestions! We will try, this weekend at macCHESS,
all those conditions for which we have reagents, and order the others now.
> On Oct 16, 2012, at 9:01 PM, "Edward A. Berry" wrote:
>
>> Would someone suggest a cryoprotectant for index screen #59? It contains
>> 0.02 M MgC
Dear Ed,
Glycerol is a solubilizing agent. Unless something is done to conteract
such effect
the crystals will dissolve. Laura Vera from my lab made a presentation at
ICCBM-14
on this subject.
We have developed a set of balanced solutions, mixed solubilizers like
glycerol and ethylene
glyc
Dear Ed,
try ca. 30 % PEG 400
Another possibility could be Perfluoropolyether Cryo Oil from Hampton Research.
This liquid doesn't mix with water. You put the crystal in few microliter and
remove the excess water droplets from your screen solution (otherwise you will
get ice rings).
Cheers,
Ul
Would someone suggest a cryoprotectant for index screen #59? It contains
0.02 M MgCl2
0.1 M HEPES pH 7.5
22% polyacrylic acid 5100, Na salt
Adding glycerol tends to dissolve the crystals.
Thanks,
Ed
