@JISCMAIL.AC.UK
Subject: [ccp4bb] control of nucleation
hello,everybody . due to excess nucleation,I often get many tiny
crystals instead of few,large crystals.i wana optimize the condition,
does anyone have adivce about this?
Best regards.
ng oil (such as parafin oil ... etc ) at reservoir
> on top of the reservoir solution. You have to try several trials to find
> optimum ratio.
>
> With regards
>
> Syed
>
> --- On *Thu, 5/6/10, zq deng * wrote:
>
>
> From: zq deng
>
> Subject: [ccp4bb] contr
Hi
I have succeeded by using oil (such as parafin oil ... etc ) at reservoir on
top of the reservoir solution. You have to try several trials to find optimum
ratio.
With regards
Syed
--- On Thu, 5/6/10, zq deng wrote:
From: zq deng
Subject: [ccp4bb] control of nucleation
To: CCP4BB
I mistakenly sent this off to Enrico, rather than to the CCP4BB. Apologies to
Enrico.
Begin forwarded message:
> From: "Charles W. Carter, Jr"
> Date: May 6, 2010 6:50:23 AM EDT
> To: est...@cea.fr
> Subject: Re: [ccp4bb] control of nucleation
>
> In fact, ther
Dear Zq Deng,
I've had success with the dilution method as described
by Dunlop and Hazes: http://scripts.iucr.org/cgi-bin/paper?en5016
For me it worked for a couple of projects, and gives you a bit more to
permutate than just varying the protein:mother liquor ratios, as Thomas
microscope and > the telescope. ~Theodore Roszak>>>> > From: zq deng > Reply-To: zq deng > Date: Thu, 6 May 2010 09:03:46 +0100> To: > Subject: [ccp4bb] control of nucleation>> hello,everybody . due to excess nucleation,I often
Roszak
From: zq deng
Reply-To: zq deng
Date: Thu, 6 May 2010 09:03:46 +0100
To:
Subject: [ccp4bb] control of nucleation
hello,everybody . due to excess nucleation,I often get many tiny crystals
instead of few,large crystals.i wana optimize the condition, does
Dear zq deng,
The standard method, I dare say, in such a case is micro seeding:
reduce the precipitant to a concentration so that you just do not get crystals
any more, wait until the drop equilibrates (about one day in the case your
precipitant is a salt, up to 5-7 days for high MW PEGs),
then
hello,everybody . due to excess nucleation,I often get many tiny crystals
instead of few,large crystals.i wana optimize the condition, does anyone
have adivce about this?
Best regards.
