Re: [ccp4bb] bfactor, freeR problems!!

2007-10-01 Thread Eleanor Dodson
Yes to keeping the same FreeR Bfactors - I doubt if it matters, but I usually start a new data set by setting Bs to the Wilson suggestion.. Twinned data - maybe you ought to use SHELXL to refine it? Eleanor john kryst wrote: Hi all !! I am working on some deletion mutants. Mutant

[ccp4bb] bfactor, freeR problems!!

2007-09-29 Thread john kryst
Hi all !! I am working on some deletion mutants. Mutants crystallized in the same space group with similar cell dimensions. My questions are the following?? 1. Is the free R flag has to be maintained across all the mutants with respect to the native. 2. Does B-factor has to be flatte