We usually try 25-30% glucose first. Weigh out 150 mg of glucose in a
microcentrifuge tube add well solution, dissolve, mix, and make up to
the 0.5 mL mark with well solution. If crystals crack in this solution,
make up a 40% glucose solution in mother liquor, and add this solution
in 0.25 drop
You can try adding 10% or 20% glycerol to your Cryo that works for us.
On Tue, 23/12/14, Yarden Opatowsky wrote:
Subject: Re: [ccp4bb] best cryprotectant
To: CCP4BB@JISCMAIL.AC.UK
Date: Tuesday, 23 December, 2014, 2:47 PM
Adding Tacsimate on top
Adding Tacsimate on top of the basic conditions worked best for us.
On Tue, Dec 23, 2014 at 11:11 AM, rohit kumar wrote:
> Dear all,
>
> I got crystals in 1.4 M sodium/potassium phosphate, ph-8.2 condition.
>
> Could anyone tell me what is the best cryprotectant for this above
> condition.
>
>
Dear all,
I got crystals in 1.4 M sodium/potassium phosphate, ph-8.2 condition.
Could anyone tell me what is the best cryprotectant for this above
condition.
Thanks
--
WITH REGARDS
Rohit Kumar Singh
Lab. no. 430,
P.I. Dr. S. Gourinath,
School of Life Sciences,
Jawaharlal Nehru University
N
