Re: [ccp4bb] a question about protein sequences in the PDB

2012-03-26 Thread Chad Simmons
The total model that fits the observable electron density should be the standard for the PDB FASTA file with the deposited structure factors, however, not all depositions contain a link to the expression construct details because many are not published, and I believe that it should be explicitly

Re: [ccp4bb] a question about protein sequences in the PDB

2012-03-26 Thread Francois Berenger
On 03/27/2012 12:20 PM, Ethan Merritt wrote: On Monday, 26 March 2012, Francois Berenger wrote: Dear list, If I take all the fasta files for proteins in the PDB, are the sequences complete? I mean, do they have holes sometimes (missing amino acids)? In theory the SEQRES records describe the

Re: [ccp4bb] a question about protein sequences in the PDB

2012-03-26 Thread Ethan Merritt
On Monday, 26 March 2012, Francois Berenger wrote: > Dear list, > > If I take all the fasta files for proteins in the PDB, > are the sequences complete? > > I mean, do they have holes sometimes (missing amino acids)? In theory the SEQRES records describe the sequence of the entity that was cryst

Re: [ccp4bb] a question about protein sequences in the PDB

2012-03-26 Thread Bosch, Juergen
I think that depends on what the depositor considered complete. Just as an example the construct you cloned say from residue 20 - 380 would you consider that complete or would you consider only the sequence complete if it contained the first 20residues ? Regarding the gaps in terms of missing res

[ccp4bb] a question about protein sequences in the PDB

2012-03-26 Thread Francois Berenger
Dear list, If I take all the fasta files for proteins in the PDB, are the sequences complete? I mean, do they have holes sometimes (missing amino acids)? Sorry for the maybe stupid question but I know that sometimes the PDB files have missing residues, I am hoping that it is not the case with t