-- Forwarded message --
From: "Roger Rowlett"
Date: Aug 10, 2013 12:01 PM
Subject: Re: [ccp4bb] Xtal formed during purification
To: "ZHIZHI WANG"
Cc:
When I worked with S. typhimurium tryptophan synthase, it would crystallize
directly from the crude ly
Dear Zhizhi,
I worked with a postdoc a while ago who also got crystals when he
concentrated his purified protein in the final step of purification. It
might be worth testing whether your crystals are of diffraction quality or
not. In my colleague's case, the rapidly growing crystals did not diffra
Dear Zhizhi,
we had a case like that. I would switch to slightly different buffer
(e.g. different pH) so crystals do not appear overnight, and then do
crystallisation screening. I bet you will have many hits in different
conditions, likely with bigger crystals.
Good luck!
Best wishes,
Tomas
On
Hi all,
After I purified my target protein by ion exchange, I left the fractions
with high protein concentrations overnight @4C. Now I saw a lot of small needle
crystals inside the EP tubes this morning.
I wonder whether there is any technique or method to get bigger crystals from
this?
ZZ
