Re: [ccp4bb] XDS Processing with Translated Detector and Radiation Sensitive Crystals

2015-04-16 Thread Jurgen Bosch
Hi Christopher, you should also limit the resolution to say 6 A on the first pass to get your correct cell parameters. Your low I/sigI suggests that the indexing did not pick up the correct orientation of the crystal. You should also see this in your INTEGRATE.LP file if you look at the spot pr

Re: [ccp4bb] XDS Processing with Translated Detector and Radiation Sensitive Crystals

2015-04-16 Thread Fischmann, Thierry
Hope this is of use. Good luck Thierry -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Christopher Barnes Sent: Thursday, April 16, 2015 4:38 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] XDS Processing with Translated Detector and Radiation Sen

[ccp4bb] XDS Processing with Translated Detector and Radiation Sensitive Crystals

2015-04-16 Thread Christopher Barnes
Hi all, I am having trouble processing synchrotron data with XDS for a large unit cell protein complex (Spacegroup P2, Cell 225 x 256 x 430). My crystals diffract to 3.1 Angstrom, but at this resolution we had significant spatial overlaps. To alleviate this we translated the detector 40 mm in b