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212-478-0698
http://www.deshawresearch.com
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf
Of Maria Håkansson
Sent: Friday, September 19, 2008 8:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Unexplained electron density
Hello D
2-478-0698
http://www.deshawresearch.com
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
Behalf Of Maria Håkansson
Sent: Friday, September 19, 2008 8:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Unexplained electron density
Hello David and others,
Thanks for y
Dear Maria --
On 19 Sep 2008, at 01:38, Maria Håkansson wrote:
Any suggestions?
In addition, could that be a water molecule present at (or near) a
special position?
Kind regards.
-- Leo --
Chavas Leonard, Ph.D. @ home
Research A
> -Original Message-
> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
> Behalf Of Maria Håkansson
> Sent: Friday, September 19, 2008 8:00 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Unexplained electron density
>
> Hello David and others,
>
> T
Hello David and others,
Thanks for yur comments.
I guess it might be as simple as water molecules, present in the
structure but not at the same time.
The density looks like a rod with uneven distribution. Both ends of
the rods (1.7-1.8 Å in between)
make hydrogen bonds to protein or other
Hi Maria,
Initial questions:
1) What's present in crystallisation/purification buffers?
2) Are any other ligand visible for the 9sigma peak?
3) Does the 9 sigma peak also have a peak in an anomalous difference map?
Assuming 1.7A is accurate (and with 1.5A resolution, you'd hope it
would be!) a me
Hello All,
I have a problem with a 9 sigma positive peak 1.7 Å away from a water
molecule (or what I believe is
a water molecule). There are several similar peaks in my map though
only one is as high as 9 sigma.
My first thought was to exclude these too close waters. However the R-
values
