Is it true that when doing twinned refinement REFMAC does not use the
assigned FreeR set?
Eleanor
Roberto Steiner wrote:
Hi Sabine,
If your question is: Is it possible to refine twinned structures with
Refmac?
My answer is: Yes. Very well.
Best wishes,
Roberto
On 20 Feb 2009, at 16:32
>
> I used phenix to assign the free reflections putting in the twin
> operators. Doing simulated annealing in phenix, I get a rather large
> difference in R/Rfree of ~7%. Well, I guess I need to do some tweaking of
> the parameters in phenix (running the latest phenix and cci_apps). When I
> tha
Hi Sabine,
If your question is: Is it possible to refine twinned structures with
Refmac?
My answer is: Yes. Very well.
Best wishes,
Roberto
On 20 Feb 2009, at 16:32, Sabine Schneider wrote:
Hi everyone,
I got good data to 2.4A on a protein-ligand complex. I want to
solve the
structure
Hi everyone,
I got good data to 2.4A on a protein-ligand complex. I want to solve the
structure by MR using a model with 45% seq. ID and 55% similarity.
Initially the data appeared to be P622 (pointless, selfrotation function
etc). Unit cell: 145 145 65, MW protein is 28kDa
I prepared the MR mo
