tRNA should be very easy to see. I suggest to buy cheap yeast tRNA as
control and test different concentrations and different dyes until you can
see it on the gel. I am not sure about SybrGold. It is a tricky dye and may
take a long time to get in. Filter and excitation both need optimization.
If
I have been a bit reluctant to reply as it's so long since I did this stuff,
but have you tried EtBr and uv to visualise the bands? I used to stain DNA gels
with crystal violet to avoid uv damage which was quite good but nowhere near as
sensitive as EtBr and uv. There are reports that you can in
